Sex pheromones are vital in facilitating the chemical communication that underpins insect courtship and mating behavior. Among female American cockroaches (Periplaneta americana), two key volatile sex pheromone components, periplanone-A (PA) and periplanone-B (PB), are predominantly released. Previous studies have indicated that PB is the primary component, but the precise interplay between PA and PB, alongside their regulatory mechanisms in male courtship and mating behavior has remained ambiguous.